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Image Search Results
Journal: PLoS Pathogens
Article Title: Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Hepatitis C Virus Replication
doi: 10.1371/journal.ppat.1003056
Figure Lengend Snippet: Huh7 cells were infected with HCV (strain Jc1) using 30 TCID 50 /cell and 48 h later cells were fixed and processed for fluorescence microscopy. In case of samples shown in panels B–D, cells were first transfected with expression constructs specified in the left of each panel and 24 h later cells were infected as described above. Samples were analyzed with a Nikon TE2000-E inverted confocal microscope at 60× magnification. (A)–(D) Colocalization of HCV proteins specified in the top of each panel with protein disulphide isomerase (PDI; an ER marker), GFP-Rab21 (marker for early endosomes), GFP-Rab7 (marker for late endosomes) or βCOP-YFP (marker for COP I vesicles). The upper panels represent a low magnification overview; boxed areas are shown as enlargement in the corresponding panel below. The nucleus was stained with DAPI (blue). Scale bars represent 10 µm (top panels) and 2 µm (lower panels). The quantification of the degree of colocalization (Pearson's correlation coefficient) is given at the top of the enlarged pictures.
Article Snippet: For image analysis we used a Perkin Elmer Ultraview ERS spinning disk on a
Techniques: Infection, Fluorescence, Microscopy, Transfection, Expressing, Construct, Marker, Staining